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Fig. 6 Role of Sorcin in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm

Journal: Cellular and molecular life sciences : CMLS

Article Title: Sorcin promotes migration in cancer and regulates the EGF-dependent EGFR signaling pathways.

doi: 10.1007/s00018-023-04850-4

Figure Lengend Snippet: Fig. 6 Role of Sorcin in cell migration after EGF and IGF treatment. Wound-healing assay in H1299 cells cultured in serum-free medium (SF) or in the presence of EGF and IGF, in control experiments (siNC) and upon silencing of Sorcin (siSRI). Images were acquired at 0 h and 24 h after each single treatment. Quantification of wound-healing assay calculated as percentage of wound closure: ((At0 – At1)/ At0) × 100), where At0 is the initial wound area and At1 is the wound area n hours after the initial scratch. Error bars indicate means ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001 as determined by Student’s t test (n = 3). Scale bars, 200 µm

Article Snippet: Western blotting was performed loading 30 μg of lysates and using the following primary antibodies: rabbit monoclonal EGFR (1:1000 in 5%BSA-T-TBS solution) (D38B1, #4267, Cell Signaling), rabbit monoclonal Phospho-EGF Receptor (1:1000 in 5%BSA-T-TBS solution) (Tyr1068, #3777 Cell Signaling, Danvers, MA, USA), rabbit monoclonal p44/42 MAPK (Erk1/2) (1:1000 in 5%BSA-T-TBS solution) (137F5, #4695 Cell Signaling, Danvers, MA, USA), rabbit monoclonal Phospho-ERK1/ERK2 (P44/ P42 MAPK) (1:1000 in 5%BSA-T-TBS solution) (T202/ Y204 # MAB-94112 Immunological Science, Italy), rabbit polyclonal CHD2 (1:1000 in 5%BSA-T-TBS solution) (#ab182013, Abcam), mouse monoclonal SLUG (A-7) (1:200 in 5%BSA-T-TBS solution) (#sc-166476 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal SNAI1 (G-7) (1:200 in 5%BSA-T-TBS solution) (#sc-271977 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal Sorcin (39-M) (1:200 in 5%BSA-T-TBS solution) (#sc-100859 Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal GAPDH (1:2000 in 5%BSA-T-TBS solution) (#TA802519, OriGene Technologies, Rockville, USA), mouse monoclonal α-Tubulin B-5–1-2 (1:3000 in 5%BSA-T-TBS solution) (#T5168, Sigma-Aldrich, Milan, Italy).

Techniques: Migration, Wound Healing Assay, Cell Culture, Control